3EY9
Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54179 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 151.370, 151.370, 153.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 2.900 |
R-factor | 0.183 |
Rwork | 0.182 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pow |
RMSD bond length | 0.007 |
RMSD bond angle | 1.129 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.007 | 0.744 |
Number of reflections | 39657 | |
<I/σ(I)> | 25.07 | 2.54 |
Completeness [%] | 98.6 | 99.6 |
Redundancy | 10.5 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 80mM potassium phosphate, 0-1% PEG 2000, 0.05-0.1% protamine sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |