3EY9
Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 151.370, 151.370, 153.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.900 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pow |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.007 | 0.744 |
| Number of reflections | 39657 | |
| <I/σ(I)> | 25.07 | 2.54 |
| Completeness [%] | 98.6 | 99.6 |
| Redundancy | 10.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 80mM potassium phosphate, 0-1% PEG 2000, 0.05-0.1% protamine sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
