3EXY
Crystal structure of the 2[4Fe-4S] ferredoxin V13G variant from allochromatium vinosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | MARREASEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.425, 51.425, 76.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.480 |
| R-factor | 0.162 |
| Rwork | 0.162 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1blu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 18180 | |
| Completeness [%] | 95.2 | 97.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 3.5M AMMONIUM SULPHATE, 0.3 M SODIUM CHLORIDE, 0.1 M TRIS , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
