3EXU
A glycoside hydrolase family 11 xylanase with an extended thumb region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.910, 51.880, 84.730 |
| Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
| Resolution | 32.760 - 1.810 |
| R-factor | 0.19259 |
| Rwork | 0.191 |
| R-free | 0.23152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bcx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.219 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.810 |
| Number of reflections | 30673 | |
| <I/σ(I)> | 16 | 3.8 |
| Completeness [%] | 87.7 | 80 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M ammonium sulfate, 0.1M MES (pH 6.5), 30% PEG mme 5000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
