3EXL

Crystal Structure of a p53 Core Tetramer Bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	298
Collection date	2008-04-19
Spacegroup name	C 1 2 1
Unit cell lengths	109.418, 68.103, 34.428
Unit cell angles	90.00, 104.18, 90.00

Refinement procedure

Resolution	57.354 - 2.200
Rwork	0.202
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.354
High resolution limit [Å]	2.200	2.200
Number of reflections	12509
<I/σ(I)>	14.7	5.6
Redundancy	4.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	200 mM lithium citrate, 20% PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Lithium Citrate
2	1	1	PEG 3350
3	1	2	Lithium Citrate
4	1	2	PEG 3350