3EXC
Structure of the RNA'se SSO8090 from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 93.479, 93.479, 48.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.670 - 2.250 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.299 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.757 |
| Number of reflections | 8834 | |
| <I/σ(I)> | 50 | 2.33 |
| Completeness [%] | 99.2 | 88.7 |
| Redundancy | 15.8 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 2 M ammonium sulfate, 0.1 M Hepes 7.5, 1% PEG550 MME plus 0.015 mg/ml chymotrypsin. Cryoprotected in 20% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
