3EWV

Crystal Structure of calmodulin complexed with a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2006-07-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	39.936, 39.936, 181.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.920 - 2.600
R-factor	0.23061
Rwork	0.228
R-free	0.28177
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lin
RMSD bond length	0.014
RMSD bond angle	1.617
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.052
Number of reflections	5611
Completeness [%]	99.0	98.4
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.7	293	27%(w/v) PEG8000, 0.1M Magnesium acetate, 0.1M Ammonium acetate, 0.05M Sodium cacodylate, pH 4.7, EVAPORATION, temperature 293K