3EWO
IBV Nsp3 ADRP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.139, 43.201, 48.940 |
| Unit cell angles | 78.02, 80.06, 73.57 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.174 |
| Rwork | 0.171 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.588 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.410 | 0.410 |
| Number of reflections | 25343 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 80.2 | 90 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.12M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 22% w/v PEG 3350, VAPOR DIFFUSION, temperature 291.0K |
