3EWN
Crystal structure of a THiJ/PfpI family protein from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.749, 63.749, 103.193 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.880 - 1.650 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.20000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.113 | 0.444 |
| Number of reflections | 29919 | |
| <I/σ(I)> | 15.8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES 7.5, 1.4M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
