3EWI
Structural analysis of the C-terminal domain of murine CMP-Sialic acid Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.090, 37.490, 78.830 |
| Unit cell angles | 90.00, 116.30, 90.00 |
Refinement procedure
| Resolution | 27.430 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25000 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.304 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.430 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.252 |
| Number of reflections | 24430 | |
| <I/σ(I)> | 27.636 | 3.4 |
| Completeness [%] | 95.6 | 69.5 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | EtOH, Tris, DTT, Neu5Ac, pH 8, vapor diffusion, sitting drop, temperature 277K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | EtOH, Tris, DTT, Neu5Ac, pH 8, vapor diffusion, sitting drop, temperature 277K |
