3EWG
Crystal structure of the N-terminal domain of NusG (NGN) from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.951, 39.951, 83.147 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.590 - 2.040 |
| R-factor | 0.19933 |
| Rwork | 0.197 |
| R-free | 0.22454 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 2EXU |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.606 | 2.100 |
| High resolution limit [Å] | 2.038 | 2.030 |
| Total number of observations | 438 | |
| Number of reflections | 5232 | |
| <I/σ(I)> | 78.6 | 17.1 |
| Completeness [%] | 97.6 | 84.1 |
| Redundancy | 8.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 29% (v/v) dioxane, 20mM Bis-Tris propane pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
