3EWE

Crystal Structure of the Nup85/Seh1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-22
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	112.559, 112.559, 350.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 3.500
R-factor	0.328
Rwork	0.326
R-free	0.36900
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX (C/D/E)
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.520
High resolution limit [Å]	3.400	3.400
Number of reflections	29579
<I/σ(I)>	13.7
Completeness [%]	96.9	98.8
Redundancy	3.1	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	289	18% PEG 3350, 0.1M Bis Tris propane, 0.2M Sodium Citrate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K