3EVQ
Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-01-31 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.097, 60.458, 37.952 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 32.080 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.202 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.139 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.460 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.301 |
| Number of reflections | 9549 | |
| <I/σ(I)> | 40.65 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.66 | 8.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
