3EVG
Crystal structure of Dengue-2 virus methyltransferase complexed with S-adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.615, 112.615, 55.696 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 2.200 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 39142 | |
| <I/σ(I)> | 12.7 | 3.6 |
| Completeness [%] | 97.8 | 95.1 |
| Redundancy | 6.69 | 6.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.2 M Ammonium sulfate, 0.65 M Lithium sulfate, 0.1 M Sodium citrate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
