3EV5
Crystal Structure of Ribonuclease A in 1M Trimethylamine N-Oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.042, 32.773, 72.807 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 29.530 - 1.680 |
| Rwork | 0.226 |
| R-free | 0.26100 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 3.620 | 1.680 |
| Rmerge | 0.059 | 0.043 | 0.321 |
| Number of reflections | 26241 | ||
| Completeness [%] | 97.1 | 96.4 | 92.4 |
| Redundancy | 2.9 | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 4000, sodium citrate, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
