3EV4
Crystal Structure of Ribonuclease A in 50% Trifluoroethanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.556, 32.827, 72.515 |
| Unit cell angles | 90.00, 90.59, 90.00 |
Refinement procedure
| Resolution | 41.120 - 1.930 |
| Rwork | 0.190 |
| R-free | 0.21900 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.203 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 4.160 | 1.930 |
| Rmerge | 0.076 | 0.052 | 0.252 |
| Number of reflections | 17788 | ||
| Completeness [%] | 98.7 | 97.9 | 98 |
| Redundancy | 3.4 | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 4000, sodium citrate, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
