3EV2
Crystal Structure of Ribonuclease A in 70% Isopropanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.868, 32.770, 72.094 |
| Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
| Resolution | 36.040 - 2.020 |
| Rwork | 0.192 |
| R-free | 0.24600 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.196 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.074 | 0.047 | |
| Number of reflections | 16257 | ||
| Completeness [%] | 51.5 | 90.5 | 1.5 |
| Redundancy | 2.6 | 3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 4000, sodium citrate, pH 5.0, vapor diffusion, hanging drop, temperature 291K |
