3EUL
Structure of the signal receiver domain of the putative response regulator NarL from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.904922 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.532, 90.038, 126.336 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 1.900 |
| R-factor | 0.21988 |
| Rwork | 0.217 |
| R-free | 0.26989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.404 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.100 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.451 |
| Number of reflections | 38512 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Tris-HCl pH 8.5, 0.2M KBr, 26% PEG 2000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
