3EUJ

Crystal structure of MukE-MukF(residues 292-443)-MukB(head domain)-ATPgammaS complex, symmetric dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 2 2 21
Unit cell lengths	58.522, 180.203, 138.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.100
Rwork	0.259
R-free	0.29100
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.210
High resolution limit [Å]	3.100	6.660	3.100
Rmerge	0.066	0.043	0.163
Number of reflections	24721
<I/σ(I)>	20.751
Completeness [%]	90.2	97.8	73.3
Redundancy	6.7	9.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7	296	Succinate, pH 7.0, hanging drop, temperature 296K
1	hanging drop	7	296	Succinate, pH 7.0, hanging drop, temperature 296K