3EUD

Structure of the CS domain of the essential H/ACA RNP assembly protein Shq1p

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	298
Detector technology	CCD
Collection date	2007-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 2
Unit cell lengths	141.938, 146.293, 48.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.416 - 2.400
Rwork	0.228
R-free	0.24800
RMSD bond length	0.010
RMSD bond angle	1.286
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM (6.0)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	90.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.067	0.046	0.398
Number of reflections	39269
<I/σ(I)>	24.621
Completeness [%]	95.2	99.5	61.5
Redundancy	6.9	7.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	0.1 M Bis-Tris HCl,0.2 M Ammonium Sulfate 25% w/v PEG 3350, pH 6.0, VAPOR DIFFUSION, temperature 298K