Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.317, 104.805, 59.854 |
| Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
| Resolution | 38.800 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3etw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.097 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.097 | 0.426 |
| Number of reflections | 13703 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 97.9 | 93.2 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M Imidazole pH 7.5, and 0.5M sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
