3ETJ
Crystal structure E. coli Purk in complex with Mg, ADP, and Pi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.800, 70.500, 88.700 |
| Unit cell angles | 90.00, 124.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.21500 |
| Starting model (for MR) | 3eth |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.207 |
| Number of reflections | 80957 | |
| <I/σ(I)> | 28.6 | 2.6 |
| Completeness [%] | 93.6 | 71.1 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | monomethylether poly(ethylene glycol) 5000, MgATP, 5-aminoimidazole ribonucleotide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
