3ESX
E16KE61KD126KD150K Flavodoxin from Anabaena
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.597, 104.142, 37.800 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 18.470 - 2.310 |
| R-factor | 0.18059 |
| Rwork | 0.176 |
| R-free | 0.23676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.760 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.036 | 0.053 |
| Number of reflections | 11284 | |
| Completeness [%] | 97.5 | 89.99 |
| Redundancy | 1.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 22% PEG 8000, 0.2M Calcium chloride, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
