3ESM
Crystal structure of an uncharacterized protein from Nocardia farcinica reveals an immunoglobulin-like fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 67.582, 67.582, 62.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.564 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.735 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.081 | 0.531 |
| Total number of observations | 53806 | |
| Number of reflections | 19837 | |
| <I/σ(I)> | 20.2 | 3.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 18.7 | 18.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.5 | 294 | 100mM citric acid pH 3.5, 2.0M ammonium sulfate, Vapor diffusion, temperature 294K |
