3ESF
Crystal Structure of the enzyme Fe-superoxide dismutase TbSODB2 from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.302, 76.504, 76.981 |
| Unit cell angles | 90.00, 92.70, 90.00 |
Refinement procedure
| Resolution | 30.180 - 2.010 |
| R-factor | 0.17721 |
| Rwork | 0.175 |
| R-free | 0.21911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gpc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.418 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 2.059 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.298 |
| Number of reflections | 52751 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 98.4 | 89.7 |
| Redundancy | 4.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.5 | 291 | 0.1 M Tris pH 8.5, 24-30% PEG4000 and 0.2 M MgCl2, hanging drop, temperature 291K |
