3ES5
Crystal Structure of Partitivirus (PsV-F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 274 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 459.266, 459.266, 459.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.960 - 3.300 |
| Rwork | 0.248 |
| R-free | 0.26700 |
| Structure solution method | AB INITIO PHASING |
| Starting model (for MR) | cryo-EM reconstruction (resolution: 7.8 A start resolution: 10.0 A) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | RAVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.206 | 0.500 |
| Number of reflections | 119427 | |
| <I/σ(I)> | 4.08 | 1.61 |
| Completeness [%] | 75.3 | 74.1 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.10 M Tris, 1.98 M Ammonium Sulfate, 0.10 M Potassium Fluoride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 283 | 0.10 M Tris, 1.98 M Ammonium Sulfate, 0.10 M Potassium Fluoride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
