3ERW

Crystal Structure of StoA from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	289
Detector technology	CCD
Collection date	2007-12-01
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	133.717, 133.717, 64.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.274 - 2.500
R-factor	0.1838
Rwork	0.183
R-free	0.20280
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BP3
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.270	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	44319
<I/σ(I)>	16.1	3.3
Completeness [%]	97.2	83.8
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	289	27% PEG 2000, 0.2 M Ammonium acetate, 0.1 M sodium acetate pH 4.8. Cryoprotected with 20 % ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K