3ERQ
Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-06-15 |
Detector | BRUKER APEX II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.978, 60.282, 37.961 |
Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
Resolution | 23.290 - 2.100 |
R-factor | 0.215 |
Rwork | 0.209 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.216 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.280 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 | 0.388 |
Number of reflections | 8216 | |
<I/σ(I)> | 20.06 | 4.49 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.45 | 11.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |