3ERO
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-01-31 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.146, 60.423, 38.117 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 27.650 - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TQO.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.419 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.420 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.055 | 0.254 |
| Number of reflections | 12137 | |
| <I/σ(I)> | 29.23 | 5.17 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.94 | 5.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, Soaked in higher % MPD before freezing, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
