3ERM
The crystal structure of a conserved protein with unknown function from Pseudomonas syringae pv. tomato str. DC3000
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937, 0.97948 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 74.971, 74.971, 213.784 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.270 - 2.450 |
| R-factor | 0.2168 |
| Rwork | 0.214 |
| R-free | 0.26892 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.610 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.103 | 0.807 |
| Number of reflections | 13874 | |
| <I/σ(I)> | 43.6 | 1.96 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 10.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 0.1M Bicine, 2.4M Ammonium Sulfate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
