3ERK
THE COMPLEX STRUCTURE OF THE MAP KINASE ERK2/SB220025
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.850, 70.000, 60.700 |
| Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ERK |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.100 | 2.000 |
| Rmerge | 0.028 * | 0.282 * |
| Number of reflections | 22843 | |
| <I/σ(I)> | 20 | 3 |
| Completeness [%] | 88.0 | 90 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | Wang, Z., (1997) Proc. Natl. Acad. Sci. USA, 94, 2327. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | 0.2 (M) | ||
| 11 | 1 | reservoir | HEPES | 0.1 (M) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | benzamidine | 1 (mM) | |
| 6 | 1 | drop | pepstasin | 0.001 (mM) | |
| 7 | 1 | drop | leupeptin | 0.01 (mM) | |
| 8 | 1 | drop | HEPES | 25 (mM) | |
| 9 | 1 | reservoir | PEG8000 | 18 (%) |
