3EQX

CRYSTAL STRUCTURE OF A FIC FAMILY PROTEIN (SO_4266) FROM SHEWANELLA ONEIDENSIS AT 1.6 A RESOLUTION

Replaces:  2QC0

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97905,0.97935
Spacegroup name	P 21 21 21
Unit cell lengths	71.210, 80.300, 141.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.553 - 1.600
R-factor	0.17
Rwork	0.168
R-free	0.19700
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.636
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.553	29.553	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.042	0.015	0.524
Number of reflections	106471	19736	21263
<I/σ(I)>	13.83	48.8	2
Completeness [%]	98.7	94.9	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	0.2M NaF, 20.0% PEG-3350, No Buffer pH 7.1, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K