3EQ2

Structure of Hexagonal Crystal form of Pseudomonas aeruginosa RssB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	76.277, 76.277, 305.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.046 - 3.401
R-factor	0.272
Rwork	0.271
R-free	0.30700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.520
High resolution limit [Å]	3.400	7.320	3.400
Rmerge	0.150	0.074	0.578
Number of reflections	26199
<I/σ(I)>	7.941
Completeness [%]	95.6	95	95.3
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	298	0.1 M Na Cacodylate, 0.2M NaCl, 4% PEG 8000, 8.2 mg/ml protein, cryo-protected with 25% glycerol , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K