3EPY
Crystal Structure of human acyl-CoA binding domain 7 complexed with palmitoyl-Coa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97243 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.515, 27.922, 60.437 |
| Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
| Resolution | 24.126 - 2.005 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cb8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.075 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.566 |
| Number of reflections | 12725 | |
| <I/σ(I)> | 8.6 | 2.6 |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 0.2 M MgCl2, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
