3EPM
Crystal structure of Caulobacter crescentus ThiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.247, 103.442, 95.369 |
| Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
| Resolution | 43.290 - 2.793 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.24400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.139 | 0.404 |
| Number of reflections | 28913 | |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 40% PEG4000, 200 mM Li2SO4, 2 mM DTT, 100 mM Tris-HCl, pH 8.2, vapor diffusion, hanging drop, temperature 298K |
