3EPE
Crystal Structure of the GluR4 Ligand-Binding domain in complex with glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.374, 105.124, 66.336 |
| Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
| Resolution | 19.270 - 1.850 |
| R-factor | 0.19222 |
| Rwork | 0.191 |
| R-free | 0.22038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.270 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 52938 | |
| Completeness [%] | 96.3 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 25% PEG 4000, 0.1M Na-Acetate pH 4.6, 0.2M Ammonium Sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
