3EP0
Methyltransferase domain of human PR domain-containing protein 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Collection date | 2008-09-15 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.882, 34.358, 103.739 |
| Unit cell angles | 90.00, 131.09, 90.00 |
Refinement procedure
| Resolution | ? - 2.100 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dal |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.287 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 21829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Purified PRDM12 was crystallized using hanging drop vapor diffusion method at 293K by mixing the protein solution with the reservoir solution containing 19% PEG 2,000 MME, 0.1 M KSCN., VAPOR DIFFUSION, HANGING DROP |
