3ENW
Substrate and inhibitor complexes of ribose 5-phosphate isomerase from Vibrio vulnificus YJ016
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.062, 77.062, 189.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.19563 |
| Rwork | 0.192 |
| R-free | 0.27181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3enq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.739 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 64147 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 8% PEG4000, 0.05M succinate, pH4.0, 0.18M ammonium sulfate, 10mM ribose 5-phosphate, pH7.5, VAPOR DIFFUSION, temperature 293K |
