3ENU
Crystal structure of Nitrollin, a betagamma-crystallin from Nitrosospira multiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-04 |
| Detector | MAR 345dtb |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 46.257, 80.903, 32.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.860 |
| Rwork | 0.181 |
| R-free | 0.21900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.348 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.10) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 4.000 | 1.860 |
| Rmerge | 0.046 | 0.030 | 0.120 |
| Number of reflections | 10654 | ||
| <I/σ(I)> | 40.15 | ||
| Completeness [%] | 99.8 | 100 | 97.8 |
| Redundancy | 6.6 | 6.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG3350, 0.2M Naformate, vapor diffusion, sitting drop, temperature 277K |
