3ENP
Crystal structure of human cgi121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 |
| Unit cell lengths | 93.521, 93.521, 87.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.610 - 2.480 |
| R-factor | 0.21336 |
| Rwork | 0.211 |
| R-free | 0.25741 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.880 | 2.580 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.049 | 0.250 |
| Number of reflections | 13866 | |
| <I/σ(I)> | 15.01 | 2.53 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.62 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% PEG 1500, 0.2 M CaOAc, 0.1 M imidazole, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
