3ENO
Crystal structure of Pyrococcus furiosus Pcc1 in complex with Thermoplasma acidophilum Kae1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 65 |
| Unit cell lengths | 66.560, 66.560, 435.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.581 - 3.020 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ivp pFu pcc1 dimeric structure |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 435.100 | 3.120 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.046 | 0.295 |
| Number of reflections | 21243 | |
| <I/σ(I)> | 14.91 | 2.41 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 1.97 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M NaCl, 40% PEG300, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
