3ENK
1.9A crystal structure of udp-glucose 4-epimerase from burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.860, 83.471, 82.576 |
| Unit cell angles | 90.00, 96.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.492 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.046 | 0.033 | 0.299 |
| Number of reflections | 73250 | ||
| <I/σ(I)> | 39.327 | ||
| Completeness [%] | 94.6 | 93.9 | 67.2 |
| Redundancy | 3.5 | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | 20% PEG 6000, 0.1M HEPES pH 7.0, 0.2M NaCl, VAPOR DIFFUSION, temperature 289K |
