3ENG
STRUCTURE OF ENDOGLUCANASE V CELLOBIOSE COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.750, 56.180, 37.370 |
| Unit cell angles | 90.00, 93.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| Rwork | 0.145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eng |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.118 |
| Number of reflections | 13151 | |
| <I/σ(I)> | 16.5 | 7.44 |
| Completeness [%] | 96.3 | 78.3 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | Davies, G.J., (1993) Nature, 365, 362. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18-22 (%) | |
| 2 | 1 | drop | protein | 12 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) |
