3EN9

Structure of the Methanococcus jannaschii KAE1-BUD32 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-02
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 2 2
Unit cell lengths	148.730, 148.730, 136.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.294 - 2.670
R-factor	0.2052
Rwork	0.202
R-free	0.26430
RMSD bond length	0.027
RMSD bond angle	1.257
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	148.730	2.760
High resolution limit [Å]	2.670	2.670
Rmerge	0.058	0.229
Number of reflections	43971
<I/σ(I)>	20.06	3.4
Completeness [%]	99.8	100
Redundancy	1.88	1.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	10 mM HEPES 10% PEG3000, 11% spermine tetrahydrochloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K