3EN2
Three-dimensional structure of the protein priB from Ralstonia solanacearum at the resolution 2.3A. Northeast Structural Genomics Consortium target RsR213C.
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 70.692, 70.692, 141.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.590 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3DM4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.239 |
| Number of reflections | 8234 | |
| <I/σ(I)> | 27.2 | 5.7 |
| Completeness [%] | 98.8 | 92.1 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 5.76M K-Acetate 0.1M Na3-Citrate, Under oil batch, temperature 277K |
