3EMP
Crystal Structure of the S-acetanilide modified form of C165S AhpC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 137.315, 137.315, 145.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.060 - 4.000 |
| R-factor | 0.302 |
| Rwork | 0.301 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yf1 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.508 | |
| Number of reflections | 13761 | |
| Completeness [%] | 98.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 1.26 M (NH4)2SO4, 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, vapor diffusion, hanging drop, temperature 277K |
