3EMI

Crystal structure of Hia 307-422 non-adhesive domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Detector technology	CCD
Collection date	2007-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785, 0.9790, 0.9750
Spacegroup name	P 63 2 2
Unit cell lengths	53.864, 53.864, 151.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.430 - 1.800
Rwork	0.162
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.881
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.098	0.270
Number of reflections	12852
<I/σ(I)>	42	11.3
Completeness [%]	99.7	99.9
Redundancy	32.6	26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	VAPOR DIFFUSION, HANGING DROP, temperature 298K