3EMF
Crystal structure of Haemophilus influenzae HiaBD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-08-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9729 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.292, 91.035, 94.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.471 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.079 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.318 |
| Number of reflections | 24079 | |
| <I/σ(I)> | 19 | 3.7 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 5.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | VAPOR DIFFUSION, HANGING DROP, temperature 298K |
