3ELZ
Crystal structure of Zebrafish Ileal Bile Acid-Bindin Protein complexed with cholic acid (crystal form A).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.93 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.195, 80.290, 39.723 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.22404 |
| Rwork | 0.222 |
| R-free | 0.26426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3elx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.462 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.347 |
| Number of reflections | 22845 | |
| <I/σ(I)> | 16.3 | 2.3 |
| Completeness [%] | 96.4 | 84.4 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1 M tri-sodium citrate dihydrate., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
