3ELY
Wesselsbron virus Methyltransferase in complex with AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.715, 66.436, 91.055 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.550 - 2.400 |
| R-factor | 0.19018 |
| Rwork | 0.187 |
| R-free | 0.25639 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3elu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.216 |
| Number of reflections | 12259 | |
| <I/σ(I)> | 18.7 | 6.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 27% PEG 4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
