3ELS

Crystal Structure of Yeast Pml1p, Residues 51-204

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9802
Spacegroup name	H 3 2
Unit cell lengths	100.613, 100.613, 102.196
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.19133
Rwork	0.189
R-free	0.23611
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	2.530
High resolution limit [Å]	1.800	2.400
Number of reflections	16896
<I/σ(I)>	19.7	1.6
Completeness [%]	99.9	100
Redundancy	6.3	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM Tris-HCl, pH 7.5, 200 mM Li2SO4, 21-24 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K